Falsely Increased Serum Estradiol Results Reported in Direct Estradiol Assays
نویسندگان
چکیده
منابع مشابه
Direct chemiluminescence immunoassay for estradiol in serum.
In this simple, reliable, fast solid-phase chemiluminescence immunoassay for directly measuring (i.e., without prior extraction) estradiol-17 beta in serum, a monoclonal antibody is used that binds estradiol with high affinity (Ka = 10(10) L/mol), and does not bind other steroids tested, the highest cross reactivity observed being 0.1% for estradiol-17 alpha. In this system the monoclonal antib...
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BACKGROUND Steroid immunoassays originally required solvent extraction, chromatography, and structurally authentic tracers to avoid interference from steroid cross-reactivity and matrix effects. The demand for steroid assays has driven assay simplification, bypassing this triplet of validity criteria to allow use of unextracted serum, which has introduced bias and nonspecificity at low steroid ...
متن کاملDirect chemiluminescence immunoassay of estradiol in saliva.
A sensitive and simple direct solid-phase chemiluminescence immunoassay is described for estradiol in saliva. In this assay, a second antibody is bound to the wells of microtiter plates. Either buffer with standards or saliva (100 microL) is incubated in these wells with monoclonal anti-estradiol antibody and with estradiol-isoluminol conjugate. Incubation time is 2 h. Chemiluminescence of the ...
متن کاملMulticenter evaluation of assays for estradiol and progesterone in saliva.
Blood samples can be difficult to obtain in studies involving serial sampling, especially in developing countries where there may also be logistic, ethical, and cultural constraints that make frequent blood collection impractical. Assays for steroids in saliva may avoid some of these difficulties. A multicenter study involving laboratories in five countries was carried out to compare the result...
متن کاملImproved Detection of Serum Estradiol after Sample
application of the cross-run rules. Data were also modeled for n 4, and the error detection for full multirules was lower than described on the Westgard site for all changes in bias from 1.0 to 3.0 times the SD when previously detected shifts were removed from the 8x rule (data not shown). The change in bias that can be detected with 90% certainty decreases from 1.8 times the SD, as shown on th...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1997
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/43.7.1250